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Nichols Institute Diagnostics
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ESUMI Co Ltd
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GenScript corporation
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CH3 Biosystems
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Dongsheng Biotech
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Novobio Scientific Inc
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Beijing CWBio
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GenScript corporation
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Image Search Results
Journal: International Journal of Oncology
Article Title: Ubiquitin-proteasomal degradation of antiapoptotic survivin facilitates induction of apoptosis in prostate cancer cells by pristimerin
doi: 10.3892/ijo.2014.2561
Figure Lengend Snippet: Overexpression of survivin reduces response to pristimerin. LNCaP and PC-3 cells were transfected with survivn expression plasmid as described in Materials and methods and overexpression of survivin was confirmed by western blot analysis (insets). Response to PM (0–5 μM) was measured in 72 h MTS assay as described in . * p<0.05 compared to control cells. Experiment was repeated three times.
Article Snippet: For expression of HA tagged-survivin, semi-confluent cultures of PC-3 cells in 60 mm 2 cell culture dishes were transfected with 10 μg of empty or
Techniques: Over Expression, Transfection, Expressing, Plasmid Preparation, Western Blot, MTS Assay